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Order NO. C100313 CAS : [58626-38-3]
3-馬來酰亞胺基苯甲酸琥珀酰亞胺酯
英文名 MBS
别名 MBS
相關類别 蛋白異源雙功能交鍊試劑 儲存 冷凍(-20℃)
分子量 314.2    
   
編 号 包裝 庫存 目錄價(¥) 您的價格(¥) 數 量
C100313-0050 50 MG 現貨
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産品描述

概述

MBS and its water-soluble analog Sulfo-MBS are heterobifunctional cross-linkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these cross-linkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

應用

MBS is not directly water-soluble and must be dissolved first in an organic solvent such as dimethylsulfoxide (DMSO) or dimethylformamide (DMF); subsequent dilution into aqueous reaction buffer is generally possible, and most protein reactants will remain soluble if the final concentration of organic solvent is less than 10%.

組份

50 mg/bottle

技術規格

Molecular Weight 314.25
Spacer arm 7.3 ?
Purity >95% on TLC
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